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BioRobotics Ltd microgrid ii tas array-spotting robot
Microgrid Ii Tas Array Spotting Robot, supplied by BioRobotics Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microgrid+ii+arraying+robot/microgrid+ii+tas+array+spotting+robot/pmc05622956-59-27-28
Average 90 stars, based on 1 article reviews
microgrid ii tas array-spotting robot - by Bioz Stars, 2026-11
90/100 stars

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Related Articles

Polymerase Chain Reaction:

Article Title: Development of a porcine skeletal muscle cDNA microarray: analysis of differential transcript expression in phenotypically distinct muscles
Article Snippet: Insert amplification of each pBK-CMV-based plasmid clone (Stratagene) was performed with T7 primer (5'-GTA ATA CGA CTC ACT ATA GGG C-3') and T3 primer (5'-CGA AAT TAA CCC TCA CTA AAG GG-3'), using a HotStarTaq Master Mix kit (Qiagen). .. PCR was conducted in a 100 μl volume, using the equivalent of 0.1 μl of miniprep plasmid (about 15 ng), for 35 cycles at 55°C for 45 s, followed by 72°C for 3.5 min, and 94°C for 1 min, with an initial activation step of 95°C for 15 min. PCR products were purified with QIAquick 96 PCR Purification kit (Qiagen) and printed onto CMT-GAPS coated slides (Corning) using a Microgrid II arraying robot (BioRobotics, Cambridge, UK). ..

Plasmid Preparation:

Article Title: Development of a porcine skeletal muscle cDNA microarray: analysis of differential transcript expression in phenotypically distinct muscles
Article Snippet: Insert amplification of each pBK-CMV-based plasmid clone (Stratagene) was performed with T7 primer (5'-GTA ATA CGA CTC ACT ATA GGG C-3') and T3 primer (5'-CGA AAT TAA CCC TCA CTA AAG GG-3'), using a HotStarTaq Master Mix kit (Qiagen). .. PCR was conducted in a 100 μl volume, using the equivalent of 0.1 μl of miniprep plasmid (about 15 ng), for 35 cycles at 55°C for 45 s, followed by 72°C for 3.5 min, and 94°C for 1 min, with an initial activation step of 95°C for 15 min. PCR products were purified with QIAquick 96 PCR Purification kit (Qiagen) and printed onto CMT-GAPS coated slides (Corning) using a Microgrid II arraying robot (BioRobotics, Cambridge, UK). ..

Activation Assay:

Article Title: Development of a porcine skeletal muscle cDNA microarray: analysis of differential transcript expression in phenotypically distinct muscles
Article Snippet: Insert amplification of each pBK-CMV-based plasmid clone (Stratagene) was performed with T7 primer (5'-GTA ATA CGA CTC ACT ATA GGG C-3') and T3 primer (5'-CGA AAT TAA CCC TCA CTA AAG GG-3'), using a HotStarTaq Master Mix kit (Qiagen). .. PCR was conducted in a 100 μl volume, using the equivalent of 0.1 μl of miniprep plasmid (about 15 ng), for 35 cycles at 55°C for 45 s, followed by 72°C for 3.5 min, and 94°C for 1 min, with an initial activation step of 95°C for 15 min. PCR products were purified with QIAquick 96 PCR Purification kit (Qiagen) and printed onto CMT-GAPS coated slides (Corning) using a Microgrid II arraying robot (BioRobotics, Cambridge, UK). ..

Purification:

Article Title: Development of a porcine skeletal muscle cDNA microarray: analysis of differential transcript expression in phenotypically distinct muscles
Article Snippet: Insert amplification of each pBK-CMV-based plasmid clone (Stratagene) was performed with T7 primer (5'-GTA ATA CGA CTC ACT ATA GGG C-3') and T3 primer (5'-CGA AAT TAA CCC TCA CTA AAG GG-3'), using a HotStarTaq Master Mix kit (Qiagen). .. PCR was conducted in a 100 μl volume, using the equivalent of 0.1 μl of miniprep plasmid (about 15 ng), for 35 cycles at 55°C for 45 s, followed by 72°C for 3.5 min, and 94°C for 1 min, with an initial activation step of 95°C for 15 min. PCR products were purified with QIAquick 96 PCR Purification kit (Qiagen) and printed onto CMT-GAPS coated slides (Corning) using a Microgrid II arraying robot (BioRobotics, Cambridge, UK). ..

other:

Article Title: Design, Validation, and Application of a Seven-Strain Staphylococcus aureus PCR Product Microarray for Comparative Genomics
Article Snippet: Modification of the standard PCR conditions was undertaken for any failures or multiple products by either adjusting the annealing temperatures or adding the PCR additive Solution Q (Qiagen) until single products of the correct expected size were obtained for all reactions.



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